Streak Plate
Streak Plate Procedure
"An inoculation loop is a tool used by microbiologists to retrieve an inoculum from a culture of microorganisms. The loop is used in the cultivation of microbes on plates by transferring inoculum for streaking."
"An inoculation loop is a tool used by microbiologists to retrieve an inoculum from a culture of microorganisms. The loop is used in the cultivation of microbes on plates by transferring inoculum for streaking."
bunsen burner
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inoculation loop
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- - Disinfect your bench top surface before beginning work. You should already be doing this before you start lab and after you are done with the lab.
- • Loops and needles need to be sterilized in the Bunsen burner flame till red hot. Allow these implements to cool before doing any inoculations to prevent killing the organism.
- • The opening of sterile media tubes and the tube of microorganism that will be used to inoculate those sterile media tubes need to be flamed after opening and before closing.
- • Flame the loop or needle after each inoculation and before a new inoculation is done. This kills any leftover organism that is still on the instrument.
- • Hold the cap of the tube with your little finger. NEVER place the cap on the bench top.
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