The term "Pure Culture" refers to a population or colony of cells growing in the absence of other species or types. The idea that is if an agar plate is inoculated with only one species and there is no contamination, then each colony on a plate will consist of genetically identical cells have have come from a single progenitor or parent cell.
Pure culture technique allows us to isolate one species from a mixed culture is a useful tool that helps to obtain a single kind of organism from a mixed culture.
There are two common methods of pure culture. Both of these methods result in individual colonies that are isolated from a mixed culture.
How to Distinginguish
• Mixed culture of the following organisms
STREAK PLATE METHOD
Pour Plate Method
Why are Petri dishes stored upside-down?
• You will need your streak plates and pour plates and three TSA slants for isolating your colonies.
- Ideally, you should see distinct separate colonies that are RED, PURPLE and WHITE.
- Isolate one of each colored colonies to its own dedicated slant tube and incubate at 25 degrees C.