- 10% formalin and 4% paraformaldehyde are the same thing. Store at room temp. Used for paraffin-embedding protocols.
- 10% formalin/ 4% paraformaldehyde + ethylene glycol and sucrose solution. Store in freezer between 4C and -20C. Use for cryosectioning. Requires epitope/antigen retrieval.
- ethylene glycol and sucrose solution. Store in freezer between 4C and -20C. Use for cryosectioning. NO epitope/antigen retrieval. May get crystalization artifacts.
Size of Tissue for Cryosections
Embedding in OCT for Cryosectioning
Suggestion – Use flat rectangular glass (such as an oversized slide (do not used charged slide) or similar shape) with rounded/firepolished edges to protect tissue from damage.
OCT being added to a plastic embedding mold for crysectioning
Formalin-Fixed Paraffin-Embedded Technique
AbCam Paraffin-Embedded IHC
HOW TO DO IHC