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  • MICROBIOLOGY UNKNOWN LAB
  • Ex 24 - Examination of Water - Most Probable Number
  • Ex 25 - Transformation

What Are Microorganisms? ​

An introduction to the field of microbiology

Find out about the history of microbiology

Microbiology literally translates as "the study of small life" in Greek. It looks at microscopic organisms, their interactions with other living organisms and with the environments. 

    Microscopic organisms are more simply called "microbes". Most microbes are unicellular and only able to be seen with the aid of a microscope. However, some unicellular microbes can be seen with the naked eye and there are multicellular organisms that are microscopic. 
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E. Coli Bacteria
    So... what is considered microscopic? Organisms measuring 100 micrometer or more (µm) are generally able  to be seen with the naked eye. The vast majority of the organisms we study in microbiology are many orders of magnitude smaller than 100 micrometers. For example, a single animal cell typically measures about 10 micrometers across, bacterial cells measure about 1 micrometer and viruses are only about 0.1 micrometers across. 
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   There are many sub-disciplines in the field of microbiology. Some of them are based on the type of organisms or agents studied.  For example, parasitology is the study of parasitic organisms. Many parasitic organisms are not microscopic, but can cause disease in humans. For this reason, parasitology is included with the other disciplines of microbiology.
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 The cell is the fundamental unit of life.  All living things are made up of one or more cells.​​

All Living Organisms can be Divided into 3 Domains as Follows:

I. Archaea
                    
  • All archaea are considered prokaryotic.
  • ​​​​​​​Used to be considered bacteria, but now seen as a distinctly different cell having features in common with both prokaryotic and eukaryotic cells. 
  • Archaea and bacteria are thought to be the first life forms on Earth 3.5 billion years ago.
​
II. Bacteria                         
  • All bacteria are considered prokaryotic. 
  • Does not have compartmentalization – no membrane-bound organelles or nucleus.
  • Archaea and bacteria are thought to be the first life forms on Earth 3.5 billion years ago.


 III.   Eukaryota or Eukarya 
  • There are 4 types of eukaryotic organisms (called kingdoms).
  1. Plants
  2. Animals
  3. Fungi
  4. Protists                                ​
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BACTERIA

     Bacteria are found in nearly every habitat on earth, including acidic deep sea vents and in frozen waters of the Arctic. Bacteria make up a significant portion of the human body. In fact, the human body is composed of approximate 10 times the number of bacteria cells that human cells! Lucky for us, most bacteria are harmless or helpful. The bacteria that are known to cause disease are called "pathogenic".
PictureThe Anatomy of a Bacterium
   While many bacteria can cause disease, most bacteria is harmless to humans or even helpful. Bacteria can cause food to rot or spoil, or it can be used to create new desirable food products like yogurt and cheese. Even though we usually give fungi all the credit for being the major decomposers of our ecosystems, bacteria play a crucial role in the environment, not only as decomposers, but as the only organisms that can perform nitrogen-fixation. Nitrogen-fixation is the ability to take carbon from the atmosphere and convert it into carbon compounds that are biologically useful. 

     Bacteria are often described in terms of their general shape; spherical (coccus), rod-shaped (bacillus), or curved (spirillum, spirochete, or vibrio). ​
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    Bacteria evolved around 3.5 billion years ago. They have various means of getting energy from the environment to live in the form of photosynthesis or chemosynthesis or are able to switch between these two modes of metabolism.

     Some examples of photosynthetic bacteria include oxygenic cyanobacteria, anoxygenic green sulfur bacteria and anoxygenic green nonsulfur bacteria. These bacteria performed photosynthesis which uses carbon dioxide and water in the presence of sunlight to create glucose and oxygen.

​     Early photosynthesizers like the cyanobacteria changed the early Earth's atmosphere to contain more oxygen. This environmental change allows for the evolution of oxygen-loving species, including humans, billions of years later.
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Anatomy of Cyanobacteria
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Archaea

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     Bacteria are not the only unicellular prokaryotic organisms. There is an entire domain of organisms called the archaea. The first archaea to be discovered were classified as bacteria. However, their physiological and morphological characteristics were so different from other bacteria cells, they were named archaebacteria and were given a seperate kingdom under the bacteria domain. The term "archaeabacteria" means "ancient bacteria". This classification was soon reorganized, making the archaebacteria into a new domain called the archaea. 
 Many archaeal species can be found in environments that are extremely hot (such as the Yellowstone hot springs), extremely salty (such as the Dead Sea), or in areas of extremely low pH (such as acid mine drainage). Adaptations to these environments are partly why they have been separated into their own domain. 

VIRUSES

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  Viruses are not technically considered to be "living organisms" according to the criteria of what characteristics an entity must have to be considered alive. There are 2 characteristics of viruses that exclude them from being classified as "alive". These are, 1) viruses are not cells, and 2) viruses do not have the machinery to reproduce without the help of a host. However, there are many scientists that would like to see a reclassification of what we consider to be "life". This id due to the incredible abilities of the virus. They are remarkable. They adapt and evolve, and they reproduce using the machinery of their host cells in ingenious ways. 

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     Viruses are considered acellular microorganisms. In other words, viruses are not composed of cells. Viruses are extremely tine and are made up of a small amount of genetic material in the form of either RNA or DNA and some proteins. They are only able to reproduce with the assistance of a host cell. The virus must invade a cell and then hijack the cell's reproductive machinery to make more copies of itself. The virus will continue to make copies of itself until the cell can no longer hold the large number of viruses. At this point the cell bursts open, releasing the viruses into the extracellular fluid where they can go invade other cells and repeat the cycle!

     Viruses can infect all types of organisms and cells. Many viruses are responsible for diseases in humans, but there are many viruses that do not lead to disease. 

​Protists

​     Protists belong to the kingdom Protista which is one of the four kingdoms of Eukarya and, therefore, are composed of eukaryotic organisms. The protista is an extremely diverse kingdom that is more easily describe as what it is NOT than what it IS. Basically, any eukaryotic organism, that cannot be classified as animalia, plantae or fungi.
     Protists are mostly unicellular organisms, but there are a few simple multicellular forms as well. Protists include algae, slime, molds, and protozoa. some protists can reproduce sexually while others reproduce asexually, some are autotrophic and can provide their own food using photosynthesis or chemosynthesis, while other protists must consume other organisms as food.
   Algae are protists that have some similar characteristics as plants (though they are not plants). These similarities include:
  1. A cell walls made of cellulose
  2. The presence of chloroplasts
  3. The ability to photosynthesize
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Photograph of Algae on Sea Bed COurtesy of... ​By Toby Hudson - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=18150674
     Algae are extremely important to the ecosystem. They photosynthesize, which means they consume carbon dioxide and create sugar and oxygen. Many organisms use oxygen and sugars created by algae. Many food-stuffs consumed by humans are made with ingredients that were derived from algae, such as carrageenan or alginic acid. These ingredients can be found in some brands of ice cream, salad dressing, beverages, lipstick, and toothpaste. A derivative of algae also plays a prominent role in the microbiology laboratory, agar. Agar is a gel;like substance derived from algae, that can be mixed with various nutrients and used to grow microorganisms in a Petri dish. Algae are also being developed as a possible source of nutrition and biofuel.
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Electron Micrograph of Gephyrocapsa oceanica courtesy of... Photo by NEON ja, colored by Richard Bartz - Own work, CC BY-SA 2.5, https://commons.wikimedia.org/w/index.php?curid=6072505
The EUGLENA are a type of algae that can be found in pond water. Click on the short video below to see how they move! They are green, because they contain chloroplasts, which allows them to make their own food using photosynthesis. They also have a flagellum which they use to move and to sense their environment. PROTOZOABlepharisma japonicum

  Protozoa

      Protozoa get their name from the Greek words 'protos', which means 'first' and "zoan", which means 'animal'. As you may have guessed, the protozoans were names this, because they were the first animal-like organisms to evolve.

     Protozoans are a very diverse group of organisms. Many of them are heterotrophic (hetero means 'other' and trophic means 'to eat'), meaning that they must consume other organisms as a food source in order to live. These protozoans usually feast on bacteria or other protozoa.

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A bdelloid rotifer
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Blepharisma japonicum
     Some species of protozoa can switch between being autotrophic (auto means 'self' and trophic means 'to eat') and heterotrophic. This means that these protozoans are capable of making their own food when there is an ample supply of sunlight, and they are capable of consuming other organisms as a food source when sunlight is scarce. There are even some protists that gain energy from the environment in a similar manner as fungi. These protists predigest their food source by secreting enzymes onto it (or into it) that function to break it down into a form that can be absorbed.
     Tardigrades (Water Bears)Tardigrades are microscopic creatures that are able to survive the some of the strangest conditions in the universe. They can hibernate without water for at least 100 years. They can survive temperatures 50% hotter than boiling water. They survive in pressures that are higher than the pressure in our deepest oceans. Tardigrades have even been blasted to outer space and were completely fine! They were just fine without air, without pressure and without water. 
Some protozoa can cause diseases. An example of an Illnesses Caused by Protozoa, is Malaria.
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     Plasmodium is a genus of parasitic protozoa, many of which cause malaria in their hosts. When the host is a mosquito, the mosquito can then spread the disease to humans.
​
     
.Symptoms include fever, vomiting, headache, jaundice, seizures or coma. Can be fatal. According to the World Health Organization, Malaria effected approximately 296 million people in 2015. Malaria is believed to have caused 730,500 of these infected people to die.

(wikipedia
) en.wikipedia.org/wiki/Malaria  ​​

Fungi


     A fungus is any member of the group of eukaryotic organisms in the KINGDOM FUNGI. This includes unicellular microorganisms such as yeasts and molds, as well as multi-cellular fungi that produce familiar fruiting forms known as mushrooms.  There are about 100,000 known species of fungi. Experts have suggested that the actual number of fungi species on Earth may be closer to as many as 1.5 million.
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​
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   Fungi are a lot of fun and can be found at most parties. Sorry for the lame joke. Bit seriously, if you do want to find fungi, the best place to look would be a warm, moist place.
   Fungi have a very interesting, or gross, way of eating. Fungi "eat" by secreting digestive enzymes directly into whatever it is they are growing on.  These digestive enzymes break down nutrients into smaller molecules that the fungi can directly absorb.



  Fungi play a vital role in the ecosystem as decomposers. They assist in recycling materials back into the soil as part of their metabolism. Some fungi have been found to have life-saving properties. For example, antibiotics like penicillin, are made from a fungus.
    Yeast is a microscopic fungus consisting of single oval cells that reproduce by budding, and are capable of converting  sugar into alcohol and carbon dioxide. Yeasts are eukaryotic, single-celled microorganisms classified as members of the fungus kingdom.

FOODS/DRINKS THAT ARE MADE POSSIBLE WITH YEAST INCLUDE...

a)    Breads
b)    Alcohols 
c)    Cakes and More!


​
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   Fermentation reaction in Yeast takes Glucose and forms ethanol, carbon dioxide and ATP. 

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     Mold grows on bread when the bread comes in contact with dust that is carrying fungi, which are tiny microorganisms. Molds are multicellular fungi. 

​     Mold spores remain airborne until they land on a piece of food, germinate and grow into a healthy fungus. Mold lives off the food its growing on by eating away at it. It commonly grows right through whatever it's eating. Fungi secrete an enzyme onto their food source that breaks it down aiding digestion. They then grow microscopic roots into the food itself.

     Molds are used in antibiotics (specifically, penicillin) and other pharmaceuticals.. Some molds can cause allergies or even diseases (mycotoxins).
Fungal Infections Cause a Number of Diseases in Humans
VAGINAL YEAST INFECTION
ATHLETE'S FOOT
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    Healthy bacteria reside in the vagina that function to regulate the growth of yeast and, thereby, help protect against yeast infections.
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Fungal Growth
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By James Heilman, MD - Own work, CC BY-SA 3.0, https://commons.wikimedia.org/w/index.php?curid=17770316
  Athlete's foot is caused by a fungal infection that invades the skin of the foot or toes. This condition can arise when the feet are exposed to excessive moisture (like sweat) for extended periods of time. 
ASPERGILLOSIS:
     Aspergillosis
 is fungal infection that is caused by the aspergillis fungus. The fungus grows in the lungs, causing inflammation and blockage of the airways. Infected patients may experience symptoms ranging from mild irritation to fever, pain and difficulty breathing.  ​
Fungus are the essential decomposers of the ecosystem! 
    They function together with certain bacteria to breakdown dead organic matter into a form that can be used in the ecosystem. For example, fungus will breakdown a dead cow and release vital nutrients into the soil. Those nutrients can then be used by plants to grow. Then another cow can go and eat the plant! ...and the cycle continues!

Helminths

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    Multicellular parasitic worms called helminths are also studied as part microbiology, even through they are large enough to see with the naked eye. This is because parasitic infections involve eggs and larvae which are microscopic.
  Some examples of helminths are
  1. The Guinea Worm, or Dracunculus medinensis, which causes dizziness, vomiting, diarrhea, and open sores. The Guinea Worm infects water fleas. The water fleas can then contaminate drinking water and pass the parasite to a human (or animal) host.  35 million cases of Guinea Worm infections were reported in the 1980's. Since the outbreak, improvements in water sanitation decreased the amount of infection to just 126 reported cases world-wide in 2014.  
  2.  Tapeworms do not have any sort of "digestive tract"! So HOW DO THESE THINGS STAY ALIVE?? WELL... tapeworms feed by living inside of the digestive tracts of other animals (even humans)!  In this way, that are able to eat the food already digested by their host!

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By SusanA Secretariat Catalina Maya Rendón - https://www.flickr.com/photos/gtzecosan/15679887846/in/ photostream/, CC BY 2.0, https://commons.wikimedia.org/w/index.php?curid=36812004
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Tapeworm Infection of a Human Host
   Tapeworms do not have any sort of mouth either. So, they eat by absorbing the nutrients they come in contact with through their skin. 
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    • FUN ZONE >
      • GAMES
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      • Population Ecology - ACTIVITY
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      • EXTRA CREDIT - The Cell Game
      • SYMBIOSIS ACTIVITY
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  • Environmental Science
    • MIDTERM 2 STUDY GUIDE
    • Exam 2 Study Guide
    • ENVS 105 Home Page >
      • Midterm 3 Study Guide Population Ecology
      • Ecology II - Communities and Ecosystems
      • Module 1 Assignments
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    • Inrtoduction to ENV SCI
    • Introduction to environmental science
    • Historical Perspective of ​Environmental Science
    • Biomes
    • ECOSYSTEMS and Energy Flow
    • FOOD CHAIN and FOOD WEB
    • Biogeochemical Recycling
    • Evolution - Our Beginning
    • Genetic Inheritance
    • Evolution: How Populations Change over Time
    • Symbiosis
    • Population Ecology
    • Competition in Nature
    • Predation
    • Herbivory
    • Niches
    • Fossil Fuels
  • Env Bio Lab
    • ENV BIO LAB FINAL STUDY GUIDE
    • The Metric System
    • Ecological Roles of Living Organisms >
      • The Basics
      • Bacteria - Ecological Roles
      • Protists - Ecological Roles
      • Fungus - Ecological Roles
      • Plantae and Animalia - Ecological Roles
    • Adaptations to Dry Climates
    • Microscopes
    • GROWTH CURVES
    • SOILS AND GROUNDWATER
    • WATER QUALITY ANALYSIS
  • Human Sexuality
    • Course Information
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    • Week 1
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    • HOME PAGE for BIO 270 Microbiology Hybrid Sections 70 & 71
    • Week 10
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  • Chemistry
    • The Chemistry of Cells - ORGANIC >
      • pH Lab
      • pH Lab
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  • MICROBIOLOGY UNKNOWN LAB
  • Ex 24 - Examination of Water - Most Probable Number
  • Ex 25 - Transformation
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